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. 2019 Nov 6;20(22):5533. doi: 10.3390/ijms20225533

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Separation of the benzo[a]pyrene and its metabolites in serum of rats. The analyses of the benzo[a]pyrene (BaP) metabolites were detected using liquid chromatography tandem mass spectrometry (LC/MS/MS). Mobile phase; D.W (0.1% formic acid): Acetonitrile (0.1% formic acid) = 2:98, calibration curve: R = 0.99, with gradient program (2:98, D.W, containing 0.1% formic acid: Acetonitrile containing 1% formic acid) as a mobile phase. Chromatographic profiles as almost baseline without the elution of BaP and metabolites, probably due to retaining the BaP and its metabolites on the resin under such conditions. Left panels indicated the typical LC/MS/MS chromatogram of (A) BaP; (B) 3-OH BaP; (C) 7-OH BaP; (D) BaP-diolepoxide (BPDE). Right panels indicated the typical LC/MS/MS chromatograms of internal standard (cryptotanshinone).