Skip to main content
. 2019 Nov 7;20(22):5554. doi: 10.3390/ijms20225554

Figure 5.

Figure 5

(A) Comparison of relative mRNA levels of genes related to mitochondrial fatty acid oxidation in mouse Dicer F/F and Dicer −/− C-MSC. The amount of mRNA was normalized using β-actin. TCA, tricarboxylic acid cycle; ETC, electron transport chain. Results are shown as mean ± SEM (n = 3), NS p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Representative MitoTracker Red CMXRos and DAPI staining of Dicer F/F and Dicer −/− C-MSC; confocal microscopy showed no remarkable change of mitochondrial mass detected by MitoTracker Red staining between Dicer F/F and Dicer −/− C-MSC (Scale bar= 50 µm). (C) Comparison of the relative amount of mitochondrially encoded 1,4-Dihydronicotinamide adenine dinucleotide (NADH): ubiquinone oxidoreductase core subunit 6 (MT-ND6) mitochondrial DNA (mtDNA) between Dicer F/F and Dicer −/− C-MSC. The amount of mtDNA was normalized to nuclear-encoded control gene DNA polymerase beta (PolB). (n = 4), * p < 0.05.