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. 2019 Nov 13;20(22):5690. doi: 10.3390/ijms20225690

Figure 2.

Figure 2

Western blot and immunohistochemistry of VSMC markers with exercise treatment and control. Marker expression levels are shown in (A,C,E) by using Western blot ((A): α-SM-actin (alpha smooth muscle actin), (C): Calponin; (E): OPN (Osteopontin)). Analysis results are shown in (B,D,F) ((B): α-SM-actin, (D): Calponin; (F): OPN). All proteins were normalized to GAPDH which serves as the referential protein. The expression levels of α-SM-actin, calponin, and OPN proteins with and without exercise treatment were measured by immunohistochemistry in (G). The analysis results are shown in (a–c) ((a): α-SM-actin, (b): Calponin, (c): OPN). Negative controls of α-SM-actin, calponin, OPN, and blank are shown in the lower part of the combination image. ## p < 0.01 (versus WKY-SED), * p < 0.05 (versus SHR-SED). Bar = 50 μm (n = 8 in each group).