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. 2019 Nov 13;20(22):5690. doi: 10.3390/ijms20225690

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression levels of VSMC protein markers after treatments with all inhibitors. Thoracic aortic was per-incubated by Akt, ERK, and p38 MAPK specific inhibitors. Western blot and immunofluorescence assays were used to test the marker proteins (α-SM-actin, calponin; OPN). Expression levels of α-SM-actin and calponin after treatment with blockers are shown in (A–D). Images (E,F) show the expression levels of OPN after treatment with these blockers. Furthermore, immunofluorescence results of marker proteins are shown in (G). ^# p < 0.05 versus WKY), * p < 0.05 versus SHR. Bar = 25 μm (n = 6 in each group).