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. 2019 Nov 13;20(22):5685. doi: 10.3390/ijms20225685

Figure 3.

Figure 3

Excision of the (CTG)2600 repeat differentially alters the temporal expression of drivers and markers of myogenic differentiation. (A) The experimental set-up for the analysis of mRNA levels for myogenic transcription factors and structural proteins before and during myoblast–myotube differentiation in cells with and without the (CTG)2600 repeat. Time points for sampling and RT-qPCR analysis are indicated (top). Anticipated changes in the expression of muscle-specific factors are depicted (bottom). (B–E) RNA expression for myogenic transcription factors (“drivers”) MYOD, MYOG, MYF5, and PAX7 (in arbitrary units). (F) RNA expression for the MEF2D + β-exon factor isoform. (G–I) RNA expression for structural muscle proteins (myogenic progression markers) DMD, MHCp, and MHCe. Each data point shows the mean ± SEM of the four different cell lines either with or without the (CTG)2600 repeat expansion. *p < 0.05, **p < 0.01, ***p < 0.001 (two-way ANOVA).