Figure 1.

The unconventional prefoldin RNA polymerase II subunit 5 interactor (URI1) promoter activation by HBx and c-MYC through E-box. (A) A reporter plasmid under the control of the URI1 promoter (−304/+67; Supplementary Figure S1A) was co-transfected into HuH7 (left) and HepG2 (right) cells with HBx- or c-MYC-expressing plasmids. (B) Reporter plasmids for the URI1 promoter with wild-type or mutant E-boxes (E-box and ΔE-box, respectively; Supplementary Figure S1) were co-transfected into HuH7 (left) and HepG2 (right) cells with HBx- or c-MYC-expressing plasmids. Luciferase assays were performed 2 days post-transfection. pCMV-Flag, and pGL4.74[hRluc/TK] were used as empty and transfection controls, respectively. Data are shown as mean ±SD (n = 3–4). #; p < 0.05 was determined by Tukey’s test.