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. 2019 Nov 11;20(22):5634. doi: 10.3390/ijms20225634

Figure 1.

Figure 1

SCN (soybean cyst nematode) juveniles; infection rate and development state assay in HPZ and W82. Each soybean seedling was inoculated with 2000 second-stage SCN juveniles, and the infection rate was assayed at 1 dpi in W82 and HPZ (A–C), A: second-stage SCN juveniles infected the root of W82, bar = 500 μm, B: second-stage SCN juveniles infected the root of HPZ, bar = 500 μm, C: total number of second-stage SCN juveniles infected the root of W82 and HPZ, values are the mean of the number of SCN, bar, standard error. The development state was assayed at 5 dpi (D–F), D: SCN juveniles developed into the swollen stage in W82, bar =200 μm, (E) the development of SCN juveniles was delayed in HPZ, bar = 200 μm, (F) total number of SCN different state juveniles in W82 and HPZ at 5 dpi, values are the mean of the number of SCN, bar, standard error. Pictures of SCN juveniles in the soybean root were taken by an OLYMPUS DP80 light microscope (Olympus, Tokyo, Japan). Nematode infection and development data were checked for normality with the Kolmogorov–Smirnov or Shapiro–Wilk test, then the t-test method was used to analyze these above data with SPSS software. Each soybean cultivar contained 10 replicates, ns: not significant, p < 0.05, p < 0.01 means a significant difference was found between each comparison.