Figure 3.

The workflow of the construction of 24 small libraries. In total, 1 mL 0.1% water-agar mixture containing approximately 2000 SCN J2s (second stage juveniles) or water-agar mixture alone as the treatment or control, respectively, were added to the root system of soybean seedlings. Three soybean seedlings were pooled as one sample, each sample contained three biological replicates, and these samples were collected at 1 and 5 dpi, respectively. In total, 24 samples used for small RNA libraries construction were prepared, namely, 24 samples = 2 (treatments) × 2 (time-points) × 2 (cultivars) × 3 (biological replicates).