Table 4.
Function annotation of the potential target genes of differentially expressed miRNAs.
| DE Set | miRNA | Target Gene | Function Annotation |
|---|---|---|---|
| W1C vs W1N | gma-miR408a-5p | Glyma.04G248700.Wm82.a2.v1 | Xylanase inhibitor N-terminal |
| gma-miR408a-5p | Glyma.06G114200.Wm82.a2.v1 | Xylanase inhibitor N-terminal | |
| gma-miR408a-5p | Glyma.07G103400.Wm82.a2.v1 | Protein tyrosine kinase | |
| gma-miR408a-5p | Glyma.09G174000.Wm82.a2.v1 | Protein tyrosine kinase | |
| gma-miR408a-5p | Glyma.10G282000.Wm82.a2.v1 | Ubiquitin-conjugating enzyme | |
| gma-miR408a-5p | Glyma.20G107300.Wm82.a2.v1 | Ubiquitin-conjugating enzyme | |
| gma-miR4415a-3p | Glyma.13G076900.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR4415a-3p | Glyma.14G041300.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR4415a-3p | Glyma.20G051700.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR4415a-3p | Glyma.20G051900.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR4415a-3p | Glyma.20G051600.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR4415a-3p | Glyma.20G051700.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR4415a-3p | Glyma.20G051900.Wm82.a2.v1 | Multicopper oxidase | |
| H1C vs H1N | novel_miR_63 | Glyma.05G145000.Wm82.a2.v1 | ABC transporter |
| novel_miR_63 | Glyma.08G101500.Wm82.a2.v1 | ABC transporter | |
| novel_miR_63 | Glyma.08G149300.Wm82.a2.v1 | Glycosyl hydrolases family 28 | |
| novel_miR_63 | Glyma.10G231100.Wm82.a2.v1 | Glycosyl hydrolases family 28 | |
| novel_miR_63 | Glyma.15G269400.Wm82.a2.v1 | Glycosyl hydrolases family 28 | |
| novel_miR_237 | Glyma.05G145000.Wm82.a2.v1 | ABC transporter | |
| novel_miR_237 | Glyma.08G101500.Wm82.a2.v1 | ABC transporter | |
| novel_miR_237 | Glyma.08G149300.Wm82.a2.v1 | Glycosyl hydrolases family 28 | |
| novel_miR_237 | Glyma.10G231100.Wm82.a2.v1 | Glycosyl hydrolases family 28 | |
| novel_miR_237 | Glyma.15G269400.Wm82.a2.v1 | Glycosyl hydrolases family 28 | |
| gma-miR3522 | Glyma.04G121700.Wm82.a2.v1 | Polyphenol oxidase middle domain | |
| gma-miR3522 | Glyma.07G193300.Wm82.a2.v1 | Polyphenol oxidase middle domain | |
| gma-miR3522 | Glyma.07G193500.Wm82.a2.v1 | Polyphenol oxidase middle domain | |
| gma-miR3522 | Glyma.13G183200.Wm82.a2.v1 | Polyphenol oxidase middle domain | |
| gma-miR3522 | Glyma.15G071200.Wm82.a2.v1 | Polyphenol oxidase middle domain | |
| gma-miR3522 | Glyma.05G167100.Wm82.a2.v1 | Neprosin activation peptide | |
| gma-miR3522 | Glyma.08G125400.Wm82.a2.v1 | Neprosin activation peptide | |
| W5C vs W5N | gma-miR408a-3p | Glyma.02G231600.Wm82.a2.v1 | Multicopper oxidase |
| gma-miR408a-3p | Glyma.02G261600.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR408a-3p | Glyma.11G164000.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR408a-3p | Glyma.11G233400.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR408a-3p | Glyma.14G056100.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR408a-3p | Glyma.14G198900.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR408a-3p | Glyma.18G023600.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR408a-3p | Glyma.18G057200.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR408a-3p | Glyma.03G189800.Wm82.a2.v1 | Leucine Rich repeats | |
| gma-miR408a-3p | Glyma.06G142500.Wm82.a2.v1 | Leucine Rich repeats | |
| gma-miR408a-3p | Glyma.19G190200.Wm82.a2.v1 | Leucine rich repeat | |
| novel_miR_178 | Glyma.04G103900.Wm82.a2.v1 | AP2 domain | |
| novel_miR_178 | Glyma.20G224000.Wm82.a2.v1 | Myb-like DNA-binding domain | |
| H5C vs H5N | novel_miR_106 | Glyma.01G031500.Wm82.a2.v1 | Aldehyde dehydrogenase family |
| novel_miR_106 | Glyma.02G034000.Wm82.a2.v1 | Aldehyde dehydrogenase family | |
| novel_miR_106 | Glyma.16G168700.Wm82.a2.v1 | Leucine rich repeat | |
| novel_miR_106 | Glyma.15G245900.Wm82.a2.v1 | Leucine rich repeat | |
| novel_miR_106 | Glyma.17G250800.Wm82.a2.v1 | Leucine rich repeat | |
| novel_miR_106 | Glyma.09G184300.Wm82.a2.v1 | Serine hydroxymethyltransferase | |
| novel_miR_34 | Glyma.01G031800.Wm82.a2.v1 | K+ potassium transporter | |
| novel_miR_34 | Glyma.02G033600.Wm82.a2.v1 | K+ potassium transporter | |
| novel_miR_34 | Glyma.04G200600.Wm82.a2.v1 | Auxin response factor | |
| novel_miR_34 | Glyma.06G164900.Wm82.a2.v1 | Auxin response factor | |
| W1N vs W5N | gma-miR159a-5p | Glyma.01G183300.Wm82.a2.v1 | NB-ARC domain |
| gma-miR159a-5p | Glyma.15G230900.Wm82.a2.v1 | NB-ARC domain | |
| gma-miR159a-5p | Glyma.06G134200.Wm82.a2.v1 | Protein kinase domain | |
| gma-miR159a-5p | Glyma.06G258300.Wm82.a2.v1 | Protein kinase domain | |
| gma-miR5037c | Glyma.01G005400.Wm82.a2.v1 | Phosphofructokinase | |
| gma-miR5037c | Glyma.04G139400.Wm82.a2.v1 | Plant calmodulin-binding domain | |
| gma-miR5037c | Glyma.20G154800.Wm82.a2.v1 | GMC oxidoreductase | |
| gma-miR5037c | Glyma.U040400.Wm82.a2.v1 | GMC oxidoreductase | |
| gma-miR5037c | Glyma.08G275900.Wm82.a2.v1 | mTERF | |
| gma-miR5037c | Glyma.08G306000.Wm82.a2.v1 | mTERF | |
| gma-miR5225 | Glyma.15G252700.Wm82.a2.v1 | Protein tyrosine kinase | |
| gma-miR5225 | Glyma.19G130400.Wm82.a2.v1 | VQ motif | |
| novel_miR_13 | Glyma.05G232000.Wm82.a2.v1 | Leucine rich repeat | |
| novel_miR_13 | Glyma.08G039400.Wm82.a2.v1 | Leucine rich repeat | |
| novel_miR_13 | Glyma.07G103400.Wm82.a2.v1 | Protein tyrosine kinase | |
| W1N vs H1N | novel_miR_120 | Glyma.01G181900.Wm82.a2.v1 | Cytochrome P450 |
| novel_miR_120 | Glyma.09G279100.Wm82.a2.v1 | Cytochrome P450 | |
| novel_miR_142 | Glyma.04G135400.Wm82.a2.v1 | Myb-like DNA-binding domain | |
| novel_miR_142 | Glyma.18G159300.Wm82.a2.v1 | Leucine rich repeat | |
| novel_miR_200 | Glyma.20G046100.Wm82.a2.v1 | NB-ARC domain | |
| novel_miR_200 | Glyma.20G046200.Wm82.a2.v1 | NB-ARC domain | |
| novel_miR_200 | Glyma.01G112600.Wm82.a2.v1 | Multicopper oxidase | |
| novel_miR_200 | Glyma.07G133900.Wm82.a2.v1 | Multicopper oxidase | |
| W5N vs H5N | gma-miR398d | Glyma.05G055000.Wm82.a2.v1 | Copper/zinc superoxide dismutase (SODC) |
| gma-miR398d | Glyma.11G236800.Wm82.a2.v1 | Multicopper oxidase | |
| gma-miR862b | Glyma.01G062400.Wm82.a2.v1 | Copper amine oxidase | |
| gma-miR862b | Glyma.01G063700.Wm82.a2.v1 | SBP domain | |
| gma-miR862b | Glyma.02G121300.Wm82.a2.v1 | SBP domain | |
| gma-miR862b | Glyma.04G254300.Wm82.a2.v1 | Serine hydroxymethyltransferase | |
| gma-miR862b | Glyma.06G107800.Wm82.a2.v1 | Serine hydroxymethyltransferase | |
| gma-miR862b | Glyma.10G036700.Wm82.a2.v1 | AP2 domain | |
| gma-miR862b | Glyma.13G123100.Wm82.a2.v1 | AP2 domain | |
| gma-miR862b | Glyma.11G166300.Wm82.a2.v1 | Glutaredoxin | |
| gma-miR862b | Glyma.11G232300.Wm82.a2.v1 | Glutaredoxin | |
| novel_miR_105 | Glyma.01G043300.Wm82.a2.v1 | WRKY DNA -binding domain | |
| novel_miR_105 | Glyma.13G365600.Wm82.a2.v1 | Glycosyl hydrolases family 17 | |
| novel_miR_105 | Glyma.15G007600.Wm82.a2.v1 | Glycosyl hydrolases family 17 |
The potential target genes of the miRNAs were predicted using TargetFinder (v1.6), and these predicted target genes were then annotated by Blast to the NR, Swiss-Prot, GO, KEGG, and Pfam databases.