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. 2019 Nov 11;20(22):5628. doi: 10.3390/ijms20225628

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The role of RgsA in GT production and virulence. (A) Determination of GT production in WT, ΔrgsA, and C′ strains. The culture supernatant of each strain was extracted with chloroform and subjected to TLC. The arrow indicates GT. (B) Survival curve of mice infected with WT, ΔrgsA, and C′ strains (n = 10/group). (C) Fungal burden in the lung tissue by RT-PCR method. (D) Lung sections from infected WT, ΔrgsA, and C′ strains were stained with Hematoxylin and Eosin (H&E) or periodic-acid Schiff (PAS). Yellow arrow indicates highly-dense fungal hyphae visualized by magenta color. H: hemorrhage; B: bronchiole; I: inflammation; N: necrosis; V: blood vessel. Scale bars = 200 µm (4×) and 50 µm (40×).