Figure 4.

Comparison of F-actin binding affinities of human N-CAP constructs. CAP binding affinities to F-actin were analyzed by high-speed (300,000× g) cosedimentation: (a) representative sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of supernatant (S) and pellet (P) fractions of N-CAP1 co-pelleted with F-actin (uncropped version of the gel is shown in Supplementary Figure S2); (b–f) binding curves with error bars representing the standard errors of the mean of three independent repetitions; (g) K_d values determined by fitting the experimental data to the binding isotherm equation defined in the Section 4.4.