Figure 5.

Effects of N-CAP oligomers on cofilin-mediated F-actin disassembly in bulk pyrene–actin depolymerization assays. Depolymerization of preassembled pyrene-labeled (10%) F-actin from the pointed ends (in the presence of CapZ) was initiated by the addition of a G-actin-sequestering drug latrunculin B along with 250 nM CFL1 (a,b) or CFL2 (c,d) in the absence of presence of 750 nM N-CAP1 (a,c) or N-CAP2 (b,d) oligomeric constructs. For clarity, error bars representing standard errors of the mean from three (CFL1) and four (CFL2) independent experiments are shown for every third data point.