Table 3.

Effects of N-CAP constructs on cofilin-mediated F-actin depolymerization rates. Initial rates of F-actin depolymerization were measured from the slopes of the pyrene–actin depolymerization curves during the first 500 seconds. Rates are expressed in nM/min as mean values with standard errors from three (CFL1) and four (CFL2) experiments.

	No CAPs	CAP1	CAP2
Actin alone	12.9 ± 2.8
CFL1	44.0 ± 4.0
CFL1 + ΔCC-N-CAP		44 ± 4.0	48 ± 6.9
CFL1 + DD-N-CAP		52 ± 4.0	52 ± 4.0
CFL1 + TD-N-CAP		60 ± 12	64 ± 4.0
CFL1 + N-CAP		72 ± 3.4	68 ± 14
CFL2	15.8 ± 6.5
CFL2 + ΔCC-N-CAP		24 ± 4.0	12 ± 1.4
CFL2 + DD-N-CAP		30 ± 0.8	48 ± 3.5
CFL2 + TD-N-CAP		42 ± 6.0	44 ± 4.0
CFL2 + N-CAP		39 ± 4.0	48 ± 2.1