AKT	protein kinase B
BSA	bovine serum albumin
CI	cell index
CSCs	cancer stem cells
DDC	liver samples from DDC (diethyl1,4-dihydro-2,4,6-trimethyl- 3,5-pyridinedicarboxylate) treated mice
DMBA	7,12-Dimethylbenz[a]anthracene
DMEM	Dulbecco’s modified Eagle’s medium
DMSO	dimethyl-sulfoxide
EMT	epithelial-mesenchymal transition
EpCAM	Epithelial cell adhesion molecule
EpICD	intracellular domain of EpCAM
FBS	fetal bovine serum
FISH	fluorescence in situ hybridization
FM	full medium control
G1/G0	G1/G0-phase of cell cycle
G2/M	G2/M- phase of cell cycle
GAPDH	glycerinaldehyde- 3- phosphate- dehydrogenase
gDNA	genomic DNA
GLUT1	glucose transporter 1
GßL	G protein β-subunit-like protein, also known as mLST8
HBV	hepatitis B virus
Hc	pericentric heterochromatin
HCC	Hepatocellular cancer
HCV	hepatitis C virus
HNF4	hepatocyte nuclear factor 4
HRP	horseradish peroxidase
LST8	mammalian lethal with SEC13 protein 8
mTOR	mechanistic (previously: mammalian) target of rapamycin
mTORC1	mTOR complex 1
mTORC2	mTOR complex 2
N-HCC25	newly established cell line isolated from murine NASH-HCC model
NAFLD	non-alcoholic fatty liver disease
NASH	non-alcoholic steatohepatitis
NTC	No template control
p-4E-BP1	phosphorylated eukaryotic translation initiation factor 4E binding protein 1
p-mTOR (Ser2448)	phospho-mTOR (Serin 2448)
p-mTOR (Ser2481)	phospho-mTOR (Serin 2481)
p-rp S6	phospho-ribosomal protein S6
P4	passage of cells number 4
PBS	phosphate buffered saline
PCR	polymerase chain reaction
PH	primary hepatocytes
PI	propidium iodide
PI3K	Phophoinositide-3-kinase
Rb	Robertsonian Fusion
Rictor	rapamycin insensitive companion of mTOR
RIPA	Radioimmunoprecipitation assay buffer
RNA	ribonucleic acid
RTCA	real-time cell analyser
S	S-phase of cell cycle
SD	standard deviation
SEM	standard error of the mean
SIRT	sirtuin
Snail	snail familiy zinc finger 1
Sox9	SRY (sex determing region) -box 9
T	unbalanced translocation
t1–3	timepoint
TACE	transarterial chemoembolization
TBS-T	tween supplemented tris-buffered saline
TICs	tumour initiating cells
Twist	twist basic helix-loop-helix transcription factor 1
wcp	whole-chromosome-painting
µCT	micro comupter-tomography