Table 3.
List of primers for polymerase chain reaction (PCR).
| Primer | Sequence | Annealing Temperature | Cycles | |
|---|---|---|---|---|
| Albumin | for rev |
TCCTGGGCACGTTCTTGTAT TGCTTTCTGGGTGTAGCGAA |
58.5 °C | 30 |
| HNF4 | for rev |
AAGGTGCCAACCTCAATTCATC CACATTGTCGGCTAAACCTGC |
60 °C | 30 |
| CK19 | for rev |
GTCCTACAGATTGACAATG CACGCTCTGGATCTGTGACAG |
57 °C | 30 |
| Sox9 | for rev |
GTGAAGAACGGACAAGCGGA GATTGCCCAGAGTGCTCGC |
60 °C | 40 |
| EpCAM | for rev |
CGGCTCAGAGAGACTGTGTC GATCCAGTAGGTCCTCACGC |
57.5 °C | 30 |
| CD44 | for rev |
CAGAGGCGACTAGATCCCTC GAGTCACAGTGCGGGAACTC |
59 °C | 30 |
| Twist | for rev |
GCCGGAGACCTAGATGTCATTG CCACGCCCTGATTCTTGTGA |
60 °C | 40 |
| Snail | for rev |
TCTGCACGACCTGTGGAAAG GTTGGAGCGGTCAGCAAAAG |
60 °C | 40 |
| GAPDH | for rev |
AGGTCGGTGTGAACGGATTTG TGTAGACCATGTAGTTGAGGTCA |
60 °C | 40 |