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. 2019 Nov 14;20(22):5700. doi: 10.3390/ijms20225700

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression and functional analysis of tricellulin knockdown in MDCK C7 cells. (a) Densitometric analysis of tricellulin protein expression levels in stable shTRIC transfectants (KD 23 and KD 24) in comparison to vector-transfected controls (Control 2 and Control 9). shTRIC leads to decreased tricellulin expression (n = 15). (b) Effect of tricellulin knockdown on transepithelial resistance (TER). Tricellulin knockdown decreases TER in MDCK C7 cells (n = 24). (c) Effect of tricellulin knockdown on ion permeability. Tricellulin knockdown increases Na⁺ and Cl⁻ permeability to the same extent in MDCK C7 cells (n = 7–16). (d) Effect of tricellulin knockdown on permeability for 4 kDa-FITC dextran (FD4). Permeability is increased in tricellulin KD 24 cells only (n = 5–10). Statistical analysis was performed using Student’s t-test between the KD clone and the corresponding control (KD 23 versus control 2, KD 24 versus control 9). (Symbols of (a), (b) and (d): Control 2: ●; KD 23: ; Control 9: ◆; KD 24: . * p <0.05, *** p < 0.001, ns = not significant).

Inline graphic — Expression and functional analysis of tricellulin knockdown in MDCK C7 cells. (a) Densitometric analysis of tricellulin protein expression levels in stable shTRIC transfectants (KD 23 and KD 24) in comparison to vector-transfected controls (Control 2 and Control 9). shTRIC leads to decreased tricellulin expression (n = 15). (b) Effect of tricellulin knockdown on transepithelial resistance (TER). Tricellulin knockdown decreases TER in MDCK C7 cells (n = 24). (c) Effect of tricellulin knockdown on ion permeability. Tricellulin knockdown increases Na⁺ and Cl⁻ permeability to the same extent in MDCK C7 cells (n = 7–16). (d) Effect of tricellulin knockdown on permeability for 4 kDa-FITC dextran (FD4). Permeability is increased in tricellulin KD 24 cells only (n = 5–10). Statistical analysis was performed using Student’s t-test between the KD clone and the corresponding control (KD 23 versus control 2, KD 24 versus control 9). (Symbols of (a), (b) and (d): Control 2: ●; KD 23: ; Control 9: ◆; KD 24: . * p <0.05, *** p < 0.001, ns = not significant).