Figure 3.

Biochemical analysis of the osteoarthritic chondrocyte pellets (long term) in the inflammatory model. a) GAG production per DNA in three different phases of the inflammatory model. b) The effect of TCM compounds on GAG/DNA ratio in human osteoarthritic chondrocytes in phase III after induction of inflammation normalized to control vehicle group (C vehicle). n = 3; n indicates the number of human OA chondrocytes donors; for each donor three experimental replicates were analyzed. For statistical analysis using Graphpad Prism, one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test (multiple comparisons) for Figure 3a and Dunnett’s post hoc test (multiple comparisons) for Figure 3b were applied. * p < 0.01, ** p < 0.001, *** p < 0.0005, **** p < 0.0001.