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a. Primers and probes (Applied Biosystems).
| Gene | Probe Type | Assay ID |
|---|---|---|
| MMP-1 | 5′ FAM-3′ NFQ | Hs00899658_m1 |
| MMP-3 | 5′ FAM-3′ NFQ | Hs00968305_m1 |
| 18s fast | 5′ FAM-3′ NFQ | Hs99999901_s1 |
MMP1: matrix metalloproteinase 1; MMP3: matrix metalloproteinase 3; FAM: Carboxyfluorescein; NFQ: nonfluorescent quencher a. Human Gene Expression Assays (ThemoFisher Scientific). b.
