Fig. 1.

Effect of CD151 deletion on de novo formation and growth of mammary tumors in the Wnt1-driven tumor model. A The Kaplan-Meier’s plot of CD151 removal and the tumor-free survival. The 3 week-old MMTV-Wnt1-expressing mice varying in CD151 genotypes, that is, wild-type (WT), heterozygous (Het or HT), knockout (KO) were subjected to the weekly examination for palpable mammary tumors. The survival time of tumor-bearing mice was calculated as the duration from the birth date to the time needed for tumors reaching 2 cm in length. Differences in survival of mice from three groups were statistically analyzed using the log-rank test. The numbers of mice being analyzed (n) and p values were indicated. B Plot of tumor size (mm³) versus time (days). The palpable tumors in individually tagged mice were measured since their initial appearance. Tumor volumes (Mean ± SEM, mm³): calculated using length-width-height × 0.52 for CD151 wild-type (WT) and knockout (KO) mice, respectively. C and D: IHC analysis of Ki-67 and caspase 3–positive cells (Mean ± SEM). Numbers of Ki67- positive cells per field were calculated for 5 mice for each CD151 genotype (C,; D, top panel)). Cleaved caspase 3–positive cells represent 1.5% and 7.5% of total cells in areas counted for WT and KO mice, respectively (D). p values were indicated. Scale bar: 50 μm.