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. 2019 Nov 15;20(22):5741. doi: 10.3390/ijms20225741

Figure 3.

Figure 3

Expression pattern analysis of SiMYB3, and over-expression of SiMYB3 in Arabidopsis increased the root growth of seeds under low-nitrogen treatment. (A) Expression profile analysis of SiMYB3 under Low Nitrogen (LN), Low Phosphorus (LP), and Low Potassium (LK) treatment conditions. Bar = 5 μm. B-E: Seeds were germinated on 1/2 MS (Nitrogen free) medium supplemented with different concentrations of Nitrogen (0.2 and 6 mM). (B) Control (6 mM N); (C) measurements of total root length; (D) LN treatment (0.2 mM N); (E) measurements of number of lateral roots. Bar = 1 cm. Each data point is the mean (±SE) of three experiments. Statistical significance was determined using Student’s t-tests (* p < 0.05, ** p < 0.01). To identify gene function, we transformed SiMYB3 into dicotyledonous model plants (Arabidopsis) and monocotyledonous model plants (rice). The expression of SiMYB3 in transgenic plants was verified by RT-PCR (Figure S2).