Figure 5.

Dynamic Change of KDM3A and KDM4C in OVX Rat Model

(A) Western blot analysis of Kdm3a, Kdm4c, and H3K9 methylation in BMSCs derived from OVX rat model (total n = 22; Sham, n = 4; OVX3w, n = 4; OVX6w, n = 4; OVX9w, n = 5; OVX12w, n = 5). The experiments were repeated for three times.

(B) The quantification analysis of Western blot results of Kdm3a, Kdm4c, and H3K9me3 show two peaks of Kdm3a and Kdm4c (n = 22). *p < 0.05, change of Kdm3a expression compared with sham; p < 0.05, change of Kdm4c expression compared with sham; #p < 0.05, change of H3K9me3 compared with sham (Wilcoxon/Mann-Whitney test).

(C) RT-qPCR assay of Ncapd2, Ncapg2, and several cell cycle inhibitor genes in BMSCs derived from OVX rat model.

Data are presented as mean ± SEM. *p < 0.05 (Wilcoxon/Mann-Whitney test, n = 22).