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. 2019 Oct 21;121(11):912–921. doi: 10.1038/s41416-019-0604-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — TPPP is a functional target of YY1. a CCK-8 assays were performed to analyse proliferation in PANC-1-YY1 (left) or BxPC-3-YY1 (right) cells transfected with TPPP-overexpressing lentiviruses or control lentiviruses. b Cell migration and invasion assays were performed. PANC-1-YY1 or BxPC-3-YY1 cells transfected with TPPP-overexpressing lentiviruses or control lentiviruses. The membranes in the chambers were stained with 0.1% crystal violet. Scale bar, 100 μm. c Wound-healing assays were performed. PANC-1-YY1 or BxPC-3-YY1 cells were transfected with TPPP-overexpressing lentiviruses or with control lentiviruses for 0 and 48 h. Magnification, ×200; scale bar, 100 μm. d HUVEC tubeformation assays were performed. Representative images of capillary-like structures stimulated by conditioned medium are shown. (*represents p < 0.05, **represents p < 0.01, ***represents p < 0.001, compared with the control group)