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. 2019 Dec 2;2:448. doi: 10.1038/s42003-019-0687-9

Table 1.

Biochemical analyses of the AtPIF3/6 variants.

Name Oligomeric statea AtPhyB-PCM interactiona,b Pfr state KD (nM)c Pr state KD (nM)c
P3.100 Homodimer + 200 ± 70 >2000
P6.100 Homodimer + 10 ± 8 n.d.
P3 Homodimer + 220 ± 40 >10,000
P6 Homodimer/monomer + 10 ± 7 n.d.
P3.fus Monomer + 270 ± 60 >10,000
P6.fus Homodimer + 200 ± 90 >10,000
P3.A Homodimer + 220 ± 40 >10,000
P6.A Monomer + 280 ± 100 >2000
P3.As Monomer + 680 ± 60 >10,000
P6.As Monomer + 710 ± 80 >10,000
P3.AA Homodimer + 370 ± 40 >3000
P6.AA Homodimer + 360 ± 40 >2000
P3.AAfus Homodimer/monomer + 230 ± 50 >10,000
P6.AAfus Homodimer/monomer + 230 ± 30 >10,000
P3.A19 Monomer >1000 n.d.
P6.A19 Monomer >2000 n.d.
P3.A14 Monomer n.d. n.d.
P6.A14 Monomer n.d. n.d.
P3.A8 Monomer n.d. n.d.
P6.A8 Monomer n.d. n.d.
P3.B Monomer n.d. n.d.
P6.B Monomer n.d. n.d.
EYFP Monomer n.d. n.d.

n.d. not detectable

aAs determined by size-exclusion chromatography

bA “+” sign indicates that an interaction could be detected by size-exclusion chromatography, a “−” sign denotes that no interaction was observed

cAs determined by fluorescence anisotropy