Table 1.
Name | Oligomeric statea | AtPhyB-PCM interactiona,b | Pfr state KD (nM)c | Pr state KD (nM)c |
---|---|---|---|---|
P3.100 | Homodimer | + | 200 ± 70 | >2000 |
P6.100 | Homodimer | + | 10 ± 8 | n.d. |
P3 | Homodimer | + | 220 ± 40 | >10,000 |
P6 | Homodimer/monomer | + | 10 ± 7 | n.d. |
P3.fus | Monomer | + | 270 ± 60 | >10,000 |
P6.fus | Homodimer | + | 200 ± 90 | >10,000 |
P3.A | Homodimer | + | 220 ± 40 | >10,000 |
P6.A | Monomer | + | 280 ± 100 | >2000 |
P3.As | Monomer | + | 680 ± 60 | >10,000 |
P6.As | Monomer | + | 710 ± 80 | >10,000 |
P3.AA | Homodimer | + | 370 ± 40 | >3000 |
P6.AA | Homodimer | + | 360 ± 40 | >2000 |
P3.AAfus | Homodimer/monomer | + | 230 ± 50 | >10,000 |
P6.AAfus | Homodimer/monomer | + | 230 ± 30 | >10,000 |
P3.A19 | Monomer | − | >1000 | n.d. |
P6.A19 | Monomer | − | >2000 | n.d. |
P3.A14 | Monomer | − | n.d. | n.d. |
P6.A14 | Monomer | − | n.d. | n.d. |
P3.A8 | Monomer | − | n.d. | n.d. |
P6.A8 | Monomer | − | n.d. | n.d. |
P3.B | Monomer | − | n.d. | n.d. |
P6.B | Monomer | − | n.d. | n.d. |
EYFP | Monomer | − | n.d. | n.d. |
n.d. not detectable
aAs determined by size-exclusion chromatography
bA “+” sign indicates that an interaction could be detected by size-exclusion chromatography, a “−” sign denotes that no interaction was observed
cAs determined by fluorescence anisotropy