Fig. 4.

Ablation of TS expression alters the invasive ability of TNBC cells. a Representative images of lung metastasis formation assay performed by injecting luciferase-expressing MDA-MB-231 cells in the tail-vein of nude mice (n = 3/group). b Quantification of the bioluminescence signal from the lung metastases (p = 0.0078, unpaired two tailed t-test). c Relative ability of TS knockdown or control cells to penetrate through a HUVEC monolayer in an in vitro extravasation assay (p = 0.00065 unpaired multiple t-test). d Sketch diagram representing the chorioallantoic membrane (CAM) assay and e quantification of control and MDA-MB-231 shTS#2 intravasation in lower CAM, 2 days after seeding in the upper CAM (p = 0.0012, multiple t-test). Experimental data are representative from at least two independent experiments with similar results. Points are avg ± SD