Fig. 6.

TS enzymatic activity is essential for maintenance of BC de-differentiation. a Quantification of deoxyribonucleotide triphosphate (dNTPs) in MDA-MB-231 cells with TS knockdown and control cells. b Reconstitution of enzymatically inactive (TS^R50C) or wild-type (TS^wt) TS in MDA-MB-231 shTS#1 cells. c Measurement of TS enzymatic activity in MDA-MB-231 shTS#1 cells expressing TS^R50C or TS^wt (p < 0.0001, unpaired two tailed t-test) (d) Proliferation (p = 0.0001, two-way ANOVA, Dunnett multiple comparison), e CD44/CD24 FACS profile, f sphere-forming ability (p < 0.0001, unpaired two tailed t-test), and g migration capacity (p < 0.0001, two-way ANOVA, Turkey’s multiple comparison) of cells that express either TS^R50C or TS^wt. Experimental data are representative from at least two independent experiments with similar results. Points are avg ± SD