Table 1.

The specification of the primers for amplification of the targeted genes

Gene name	Primer sequence (5′ to 3′)	Accession number	Position	Size (bp)	Tm (°C)	Optimized annealing temperature (Ta) (°C)
IL-6-F	GTTCGGATAATGTAGCCT	NM_001003301.1	633–650	135	40.6	53.9
IL-6-R	TCACAGAGAACAACATAACT		751–768		40.5
CCL-2-F	GTGATCTTCAAGACCGTCCTAA	NM_001003297.1	191–212	130	47.9	59.5
CCL-2-R	TTCAGAGTGAGTATTCATGGCTT		299–321		46.6
IFN-β-F	AAACTTCACCTGGGACAA	NM_001135787.1	390–407	118	40.6	55.9
IFN-β-R	TTTCTGCTTGGACTATTGT				39.5