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. 2019 Oct 10;216(12):2778–2799. doi: 10.1084/jem.20190147

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© 2019 Lam et al.

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Figure 5. — p.R186C is associated with impaired NK cell cytotoxic function. (A) Assessment of NK cell cytotoxicity in Pt 2 and 3. Using NK cell cytotoxicity assays, Pt 2 at diagnosis had a decreased cytotoxic function compared with an HD at the shown E/T ratios at time points 2 h and 3 h. For Pt 3 at diagnosis, NK cells were stimulated with IL-2, and cytotoxicity was also decreased. (B) Functional characterization of YTS NK cell model. Standard Cr-51 release assay of YTS NK cell lines against 721.221 target cells. The mutant cell line showed a significant decrease in cytotoxicity (pooled mean ± SD, n = 3 independent repeats each with triplicates; ***, P < 0.001, Mann–Whitney U test). (C) Co-culture conjugation assay of YTS NK cell lines against 721.221 target cells. Compared with parental YTS NK cells, a reduced ability of the YTS NK cells expressing the mutant allele to form conjugates with 721.221 target cells was also observed. A representative figure is shown (n = 4).