Figure 3.

CRISPR/Cas9-mediated MDM2 knockout blocks SP141's activity in HCC cells. HepG2 and Huh7 cell lines with or without CRISPR/Cas9-mediated MDM2 knockout (sgMDM2) were exposed to various concentrations of SP141 for (A) 24 h for analyses of protein expression or (B) 72 h for cell viability measurement via MTT assays. (C) These cells were then treated with or without SP141 (0.05 μM) for 24 h, followed by measurement of the cell invasion via transwell cell invasion assays. All assays were performed in triplicate and repeated three times. (*P < 0.05 and ^#P < 0.01).