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. 2019 Dec 2;9:18118. doi: 10.1038/s41598-019-54741-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AGS increases the relative amount of NeuN positive cells, and protects from neurodegeneration. (a) Immunofluorescent staining using DAPI and NeuN after 48 hours of the indicated various treatments compared to an untreated control. Slides were visualized using a panoramic slide scanner (Panoramic Midi II scanner 3DHISTECH, Budapest, Hungary). Scale bar: 500 μm.The enlarged images were obtained using the sniping tool software for the selected area in the pictures (b) Cell size distribution. Cell size was determined using CellProfiler software. Cell sizes were divided into 3 groups of arbitrary size. Cells with a size smaller than 100 pixels (<100), a size of between 100 and 200 pixels (100–200) and cells larger than 200 pixels (>200). (c) Relative amount of NeuN positive cells. Normalized to total amount of DAPI stained cells. (Mean ± SEM, n = 5), ANOVA test *p < 0.05, **p < 0.01.