Fig. 3.

LBR regulates the NF vs CAF breast fibroblast phenotype. a, b Immortalised breast NFs (left) or CAFs (right) were transfected with siRNA targeting LBR (siLBR) or control (NC), or plasmid to allow overexpression of LBR (LBR OE) or control plasmid (pCDNA), and LBR expression was assessed using qPCR (a) or western blots (b). qPCR data represent biological triplicates, while β-actin represents a loading control for the western analysis. c–e Immortalised breast NFs (c, e) or CAFs (d, e) were transfected as shown, and the relative expression levels of the CAF marker genes α-SMA, fibroblast-specific protein (FSP), CCL2 and VEGF were determined by qPCR (c, d), or of α-SMA and vimentin by western blot (e). qPCR data represent biological triplicates, while β-actin represents a loading control for the western analysis. ***p < 0.0005 and **p < 0.005