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. 2019 Dec 2;10:5479. doi: 10.1038/s41467-019-13448-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Inactivation of AtNOP2A gene leads to the establishment of a new shorter telomere length set point. a Representation of the Arabidopsis NOP2A/OLI2 gene model. Colored boxes represent exons; black lines, introns; and black boxes, untranslated regions. Positions of T-DNA insertion sites and the corresponding mutant line numbers are indicated. Predicted S-adenosyl-l-methionine-dependent methyltransferase functional domains are indicated on top. b A representative TRF southern blot for Sf-2 and Col-0 wild types and two NOP2A/OLI2 homozygous T-DNA mutant lines (nop2a-2 and nop2a-3) is shown, where mean telomere length (mean TRF) for each individual analyzed plant is indicated at the top. Molecular weight DNA markers (in kb) are shown on the left. c The distributions of telomere length in ≥3 biological replicates of the Col-0 wild type and the two homozygous mutants are shown in boxplots (midline = median, box = interquartile range (IQR), whiskers = 1.5*IQR). Across generations, T-DNA mutants show shorter telomere length set point than the corresponding wild type Col-0 genotype. d An example of a TRF southern blot for telomere length analysis of several consecutive generations of homozygous nop2a-4^−/− mutants. Seeds of a single self-pollinated heterozygous nop2a-4^+/− plant were grown to identify wild type, heterozygous and homozygous G1 progeny, which were then used to generate corresponding G2 and G3 mutant generations by single seed descent. e, T-DNA mutants with the null nop2a-4 allele displayed significantly shorter telomeres after only a single generation of self-pollination, but established a new stable set point by the third generation of mutants.