Fig. 3.
Silencing of TBC1D24 impairs AIS formation. a Representative images of transfected GFP-positive PCNs labelled for the AIS markers Ankyrin G (grey) and pan NaV (red) at 10 DIV. White arrows indicate immuno-positive AIS tracts. Scale bar, 20 µm. b Quantification of the number of Ankyrin G and pan NaV-positive cells expressed in percent on the total number of GFP-positive cells. Data are means ± SEM from 46 and 54 fields for Scr-TBC and Sh-TBC respectively, from 3 independent preparations. c High magnification of cells treated as in A. Scale bar, 5 μm. d Quantification of the Ankyrin G and pan NaV fluorescence intensity in immuno-positive control and TBC1D24-silenced neurons. e Comparative measurements of the AIS start, maximum and end distances from the cell body performed for both Ankyrin G (left) and pan NaV (right) immunostainings in control and TBC1D24-silenced neurons. Data are expressed as means ± SEM from 92-61 Ankyrin G and 71-13 pan NaV for Scr-TBC and Sh-TBC, respectively, in 3 independent preparations. Among GFP-positive neurons, Student’s t test: *p < 0.05; **p < 0.005; ****p < 0.0001