Fig. 5.

Silencing of TBC1D24 leads to an axonal projection defect. a Representative coronal cortical section from P7 rat brain electroporated at E15 with GFP. Boxes show areas used to calculate fluorescence intensity of neurons in the somatosensory cortex (Cx) and their projections extending toward the corpus callosum (cc). Scale bar, 1 mm. b Representative P7 somatosensory cortical sections showing GFP-positive cells in brains electroporated at E15 with the indicated plasmids. Scale bar, 500 µm. c High magnification of cc bundle under the same experimental conditions as in B showing GFP signal, SMI-312 labellling and their merge. Scale bar, 100 µm. d Quantification of the cc axon bundle GFP fluorescence intensity normalized to the respective GFP intensity in the ipsilateral Cx. Data are means ± SEM from 6 (Scr-TBC), 5 (Sh-TBC) and 6 (Sh-TBC + TBC1D24) brains obtained from two independent electroporation. *p < 0.05, **p < 0.001; Kruskal–Wallis/Dunn’s tests