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PTP activity measurements using SAMDI. (A) The phosphopeptide substrate and the corresponding dephosphorylated product that are present in a reaction mixture are captured onto a maleimide-presenting self-assembled monolayer. (B) Representative SAMDI spectra from wells containing 0 cells (top) and 1 cell (bottom). Blue star: Adduct peak of substrate. Green star: Adduct peak of product.
