Fig. 3.

The PTK2 inhibitor defactinib recovers EGFR-TKI sensitivity, but not pemetrexed sensitivity, in PC-9/PEM. a Immunoblots of phosphorylated and total PTK2 in PC-9 and PC-9/PEM cells treated with defactinib at the indicated concentrations for 96 h. b Immunoblots of the phosphorylated and total levels of the indicated proteins in PC-9 and PC-9/PEM cells treated with 3 μM defactinib or DMSO for 96 h. c Viabilities of PC-9 and PC-9/PEM cells treated with defactinib at the indicated concentrations for 96 h. Data are means (SD), n = 3. *, P < 0.05; **, P < 0.01; ****, P < 0.0001. Data for treated and untreated cells were compared by one-way ANOVA and Tukey’s HSD multiple comparisons test. d-f Viabilities of PC-9/PEM cells either with or without defactinib and treated with pemetrexed (d), erlotinib (e), or osimertinib (f) at the indicated concentrations for 96 h. Data are means (SD), n = 3. *, P < 0.05; **, P < 0.01. d Data were compared by one-way ANOVA and Games–Howell multiple comparisons test. e and f Data were compared by Student’s t-test