Fig. 2.

TEC have reduced rates of proliferation and ribosomal biogenesis with age. a Representative flow plots of the frequency of BrdU⁺ cells in cTEC (CD45⁻EpCAM⁺Ly51⁺UEA⁻) (above) and mTEC (CD45⁻EpCAM⁺Ly51⁻UEA⁺) (below) displayed as histograms (black line) overlaid on the negative control (gray bar). Bar graphs represent the frequencies of BrdU⁺ cTEC and mTEC populations. b A row normalized heatmap of expression of KEGG ribosomal genes at indicated ages, where each column represents an average of a minimum of three biological replicates. c Representative flow plot of Pyronin Y staining on DAPI⁻CD45⁻EpCAM⁺ total TEC from indicated timepoints, overlaid as a histogram, where gray filled histogram is RNAse treated adult cells. Bar graph represents the MFI of Pyronin Y at each indicated timepoint, relative to the Pyronin Y MFI of the adult sample which is set to 1 and so has no error bar. All bar graphs show mean ± SEM for a minimum n = 3 mice per age. A two-tailed unpaired Student’s t test was performed to determine significance. *p < 0.05, **p < 0.01, ****p < 0.0001. The source data underlying (a, c), are provided as a Source Data file.