Fig. 7.

Transgenic Myc expression in adult TEC confers fetal properties and can drive thymic regeneration. a Schematic of the experimental design of intrathymic injections. b Representative flow plots of the frequency of CD45⁻EpCAM⁺ total TEC recovered from WT hosts receiving adult WT or FoxN1MycTg donor TEC. c Bar graphs display total cells in thymus (left) and CD45⁻EpCAM⁺ total TEC counts (right) from WT host thymi receiving WT (white) or FoxN1MycTg (gray) donor TEC. Bar graphs show mean ± SEM, where circles display experiment 1 and squares display experiment 2. d A schematic of the experimental design of tamoxifen induced Myc expression in TEC. e Representative flow plots of the frequency of CD45⁻EpCAM⁺ total TEC recovered from K5⁻MycTg⁺ (WT) or K5⁺MycTg⁺ (iMycTg) tamoxifen treated mice. f Bar graphs display total thymus counts or CD45⁻EpCAM⁺ total TEC counts from K5⁻MycTg⁺ (white) or K5⁺MycTg⁺ (gray stripe) tamoxifen treated mice. Bar graphs show mean ± SEM for a minimum n = 2 mice per genotype. A two-tailed unpaired Student’s t test was performed to determine significance. *p < 0.05, **p < 0.01, ****p < 0.0001. The source data underlying (c, f) are provided as a Source Data file.