Fig. 4.

H2AX and p53 phosphorylation in olaparib-treated A549-CRISPR-ATM cells is DNA-PK-dependent. a A549-CRISPR-ATM cells were incubated with DMSO alone (lane 1) or olaparib (4 µM, lanes 2–10) in the absence or presence of the ATR inhibitor (VE-821, 1 µM, lanes 5–7) or the DNA-PK inhibitor (NU7441, 1 µM, lanes 8–10) as shown in lanes 5–7 and 8–10, respectively. Cells were harvested after 1, 2 or 4 days as indicated, and aliquots were run on SDS-PAGE with immunoblot as in Fig. 3. Quantitation of three separate repeats is shown in (b–f). See also Supplementary Fig. 8 for additional experimental repeats. Untreated cells are shown by the hatched bar in the first lane on the left (see Supplementary Fig. 8 for additional details). All panels show the mean with SEM from three independent experiments. One-way ANOVA was used to determine the statistical significance of three separate experiments. * indicates p-value < 0.05 for VE-821 + olaparib or NU7441 + olaparib treatment groups when compared with olaparib alone