Fig. 2.

Proximal chromatin state transition reflects asynchronous cell fate commitment of intraembryonic lineages. a Average genomic coverage, histone mark probabilities, chromatin state definitions, genomic annotation overlap, average DNA methylation level and enrichment of non-coding mammalian conserved elements. b, d, f Bar plots showing the ratio of 200-bp bins with respective chromatin state changes along with gene expression changes during the development of each intraembryonic lineage at the promoter regions (ectodermal lineage (b); mesendodermal lineage (d); mesodermal lineage (f)). The promoter regions (TSS ± 2 kb) were divided into twenty 200-bp bins, and bins with the same chromatin state change were summed to calculate the percentage. Representative chromatin state changes are highlighted with rectangles and related genes are also listed. c, e, g Representative genome browser snapshots of H3K4me3, H3K27ac and H3K27me3 at the loci of representative genes (highlighted in red in b, d, f). H3K27me3 ChIP-seq and DNA methylation data for the mouse gastrula are from our previous report (GSE104243)