Fig. 5.

Identification of gastrula-specific enhancers. a Heatmap showing a large number of gastrula-specific enhancer bins based on comparison with the published dataset from ENCODE. The dataset used is also listed in Supplementary information, Table S4. b Representative biological process terms and expression stage information for selected enhancer bin clusters based on GREAT analyses. c‒e Representative genome browser snapshots of H3K27ac for gastrula-specific enhancers during development of the ectoderm (c), mesendoderm (d), and mesoderm (e). f Representative genome browser snapshot of H3K27ac for Mes1 enhancer in the gastrula and later embryonic samples. g Enhancer activity reporter expression (blue staining) in representative transgenic mouse gastrula (E7.5) and E8.5 stages. A cryosection slide of the stained E7.5 embryo is also shown. H3K27ac data of E11.5 and P7 (postnatal day 7) embryo samples are from a published dataset¹⁹