Fig. 6.
Vital organ development-related enhancers are pre-marked by H3K27ac in the gastrula. a–c Alluvial plots revealing the global dynamics of enhancer bins during the development from the 8-cell stage embryo to primary germ layers in the gastrula and related future organs. Ectodermal lineage and fetal brain (a); mesendodermal lineage and fetal liver or fetal heart (b); mesodermal lineage and fetal heart (c). Numbers of enhancer bins for each state are shown. The related biological process terms of neighboring genes of selected enhancer clusters are shown in the middle panel. Genome browser snapshots of H3K27ac and H3K4me1 around representative genes are shown. Enhancer regions with relevant dynamics are highlighted with red rectangles and enhancer activity reporter expression (blue staining) for highlighted enhancer regions is also shown on the right. Enhancers for Nkx2-5 and Notch1 were named Men1 and Men2, respectively. H3K27ac data of E11.5 and P7 embryo samples are from a published dataset19