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. 2019 Dec 2;9:18130. doi: 10.1038/s41598-019-54544-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative RT-PCR results showing the fold changes in the expression of the (a) gata1 and (b) tp53 genes in rpl10a MO^aug, MO^sp, homozygous mutant, and MO^sp + mRNA injected embryos at 24 hpf compared to wild-type and rps19 knockdown embryos as controls. (c) Fold changes in the expression of hbae3 and hbbe1 mRNA in Rpl10a-deficient embryos and mutant samples at 48 hpf. Wild-type embryos were used as controls. Each group used 20 pooled embryos (replicates = 4). The data were analyzed for statistical significance by one-way ANOVA followed by Tukey’s multiple comparison test. (**p-value > 0.01).