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. 2019 Dec 2;9:18132. doi: 10.1038/s41598-019-54556-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of hydroxychloroquine on C. burnetii growth at different pH. In (a), C. burnetii were exposed to the indicated concentrations of hydroxychloroquine and no effect on growth was observed. In (b), hydroxychloroquine was added at 10 μg/ml to all of the cultures and doxycycline was titrated. The ratios of the means of genomes at day 7 to the means of genomes at the beginning of the culture were calculated to determine fold change in genomic equivalents. The standard errors of the means of genome equivalents were calculated and used to determine the upper and lower bounds of the ratios (fold change). The error bars depict the upper and lower bounds of fold change for each condition.