Fig. 5.

Eribulin-induced Slug expression correlates with endogenous Smad4 expression. a Western blot analysis of whole-cell lysates from 12 breast cancer cell lines. Immunoblots were probed for Smad2/3, Smad4, Snail and Slug. As a loading control, a total protein stain using the REVERT™ reagent is shown along with normalised lane quantifications. b Breast cancer cell lines were serum-starved for 18 h and treated with either vehicle, eribulin or paclitaxel for 4 h. qRT-PCR analysis was performed to evaluate the mRNA fold-change of SNAI2. Data are the average of two independent experiments in the form of a heat-map. c Whole-cell lysates from BT-549, MDA-MB-468 and Smad4-expressing MDA-MB-468 cells were evaluated for Smad4 and GAPDH by immunoblotting. d MDA-MB-468 and Smad4-expressing MDA-MB468 cells were serum-starved for 18 h and treated with either vehicle or eribulin for 4 h. SNAI2 mRNA was analysed using qRT-PCR. Data are the average of two independent experiments (±SEM). Results were compared to un-transfected and drug-treated MDA-MB-468 cells shown in Fig. 5b, control