Fig. 3.

Combination of Api and TG induces caspase activity. Caspase-8 (Casp-8) and caspase-9 (Casp-9) were silenced in HCT116 cells using shRNA. Mock shRNA was used as control. a, b Caspase-3 activities were measured in mock and caspase-8 and caspase-9 silenced cells following treatment with Api or TG alone or their combination. Whole cell lysates were used to analyse caspase-8 and caspase-9 knockdown using western blotting. Actin serves as a loading control. Data are mean ± SD (n = 3). *p < 0.05 vs respective controls/groups