Fig. 4.

Bax and IL-8 are inversely correlated in prostate and colon cancer cells. HCT116 cells were treated with Api and TG alone and in combination. The level of IL-8 was determined in lysate (a) and supernatant (b) using ELISA. Mock and Bax reconstituted DU145 cells were treated with Api and TG alone and in combination. (c) Bright field microscopy images of control, Api, TG and Api plus TG treated mock and Bax reconstituted DU145 cells. d IL-8 was quantified by ELISA using DU145 whole cell lysates. e IL-8 was quantified using whole cell lysates isolated from HCT116 WT and Bax knockout cells. Data are mean ± SD (n = 3). *p < 0.05 vs respective controls