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. 2019 Dec 2;10:5486. doi: 10.1038/s41467-019-13114-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Oxidative stress-regulated phosphoproteome in adipocytes. a Experimental design of the phosphoproteomic analysis of insulin- and BCNU/AF-treated adipocytes. b Principal component analysis of the phosphoproteome. c Summary of oxidative stress-regulated phosphoproteome in adipocytes with or without insulin treatment. d Volcano plot showing oxidative stress-regulated phosphoproteome in adipocytes with 2 h BCNU/AF treatment in the presence of insulin. Significantly regulated phosphosites (by moderated t-test from limma R package) were indicated in red (up-regulated) or blue (down-regulated, adjusted p < 0.05 and absolute fold change > 1.5). e Gene ontology over-representation analysis (Fischer’s exact test) of significantly oxidative stress-regulated phosphoproteins with 2 h BCNU/AF treatment in the absence of insulin.