Table 1.
Photophysical rate parameters, together with 95% confidence intervals, determined for NBD-PC12 in POPC SUVs using TRAST.
| Base rate μs−1 |
O2 μs−1 mM−1 |
16-DOXYL μs−1 (mol%)−1 |
TEMPO-PC μs−1 (mol%)−1 |
|
|---|---|---|---|---|
| kisc | 5.7 ± 0.7 | 1.8 ± 0.3 | 1.4 ± 0.2 | 1.0 ± 0.2 |
| kT | 0.041 ± 0.007 | 1.6 ± 0.2 | 0.11 ± 0.02 | 0.22 ± 0.04 |
| kox | 0.0007a | |||
| kred | 0.0005a |
The confidence intervals consider both the global fitting of the TRAST curves as well as the subsequent maximum likelihood estimation of the bimolecular quenching coefficients.
aDetermined within one order of magnitude.