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. 2019 Dec 2;9:18037. doi: 10.1038/s41598-019-54608-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Number of yeast cells and PBMCs per ml determined by a haemocytometer, at magnification of 400x, after different cell lysis methods. Error bars represent the standard deviations of results from six biological replicates. Statistically significant differences are marked with one (p < 0.05) or two (p < 0.005) asterisks (Friedman test) and with one bracketed asterisk (p < 0.05) (Wilcoxon test).