Table 1.

Effect of different enrichment methods on PCR-based amplification^a of human DNA, fungal DNA, human RNA and fungal RNA.

Human DNA	Cq valueb	SD	Log cells/mlc	CV	DNA %c	CV
No enrichment	25.31	0.46	5.99	0.02	100.00	0.26
Buffer RLT	35.89**	0.59	2.96	0.06	0.09	0.32
Triton X-100	26.00	0.28	5.79	0.01	63.82	0.17
Bead beating	27.84*	1.62	5.27	0.09	18.93	0.66
Fungal DNA	Cq value	SD	Log cells/mlc	CV	DNA %c	CV
No enrichment	23.20	0.22	6.05	0.01	100.00	0.13
Buffer RLT	23.50	0.47	5.97	0.02	83.08	0.25
Triton X-100	23.87	0.66	5.87	0.03	66.03	0.34
Bead beating	23.60	1.62	5.94	0.07	77.91	0.63
Human RNA	Cq valueb	SD	Log cells/mlc	CV	RNA %c	CV
No enrichment	30.17	0.37	6.13	0.02	100.00	0.20
Buffer RLT	35.00**	0.61	4.85	0.03	5.20	0.31
Triton X-100	34.53**	0.60	4.98	0.03	6.93	0.31
Bead beating	34.25**	1.05	5.05	0.06	8.20	0.48
Fungal RNA	Cq valueb	SD	Log cells/mlc	CV	RNA %c	CV
No enrichment	20.25	0.48	5.93	0.03	100.00	0.29
Buffer RLT	20.97	0.22	5.71	0.01	59.94	0.14
Triton X-100	37.70**	0.77	0.52	0.46	0.00	0.42
Bead beating	34.02**	0.82	1.66	0.15	0.01	0.44

^aHuman nucleic acids amplified with CXCL-1 primers and fungal nucleic acids amplified with ITS-1 primers.

^bCq values are means of six biological replicates composed of mixes of 10⁶ PBMCs and 10⁶ Candida cells. Statistically significant differences are marked with one (p < 0.005) or two asterisks (p < 0.001) (Linear mixed model).

^cLog cells/ml, DNA percentage and RNA percentage were calculated by extrapolation of Cq values with a standard curve.

SD: Standard deviation. CV: Coefficient of variation.